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The Resonance Raman (RR) spectra of basal cell carcinoma (BCC) and normal human skin tissues were analyzed using 532nm laser excitation. RR spectral differences in vibrational fingerprints revealed skin normal and cancerous states tissues. The standard diagnosis criterion for BCC tissues are created by native RR biomarkers and its changes at peak intensity. The diagnostic algorithms for the classification of BCC and normal were generated based on SVM classifier and PCA statistical method. These statistical methods were used to analyze the RR spectral data collected from skin tissues, yielding a diagnostic sensitivity of 98.7% and specificity of 79% compared with pathological reports.
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VRR spectroscopy was used for BCC and normal skin tissues with 532nm excitation. The spectra showed significant changes in collagen, carotenoids and lipids. These enhanced fingerprints demonstrate a potential use as label-free pathology method. © 2018 The Author(s).
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- Conference Paper (2)