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Human mesenchymal stem cells (hMSCs) are multipotent progenitor cells with the potential to differentiate into various cell types, including osteoblasts, chondrocytes, and adipocytes. These cells have been extensively employed in the field of cell-based therapies and regenerative medicine due to their inherent attributes of self-renewal and multipotency. Traditional approaches for assessing hMSCs differentiation capacity have relied heavily on labor-intensive techniques, such as RT-PCR, immunostaining, and Western blot, to identify specific biomarkers. However, these methods are not only time-consuming and economically demanding, but also require the fixation of cells, resulting in the loss of temporal data. Consequently, there is an emerging need for a more efficient and precise approach to predict hMSCs differentiation in live cells, particularly for osteogenic and adipogenic differentiation. In response to this need, we developed innovative approaches that combine live-cell imaging with cutting-edge deep learning techniques, specifically employing a convolutional neural network (CNN) to meticulously classify osteogenic and adipogenic differentiation. Specifically, four notable pre-trained CNN models, VGG 19, Inception V3, ResNet 18, and ResNet 50, were developed and tested for identifying adipogenic and osteogenic differentiated cells based on cell morphology changes. We rigorously evaluated the performance of these four models concerning binary and multi-class classification of differentiated cells at various time intervals, focusing on pivotal metrics such as accuracy, the area under the receiver operating characteristic curve (AUC), sensitivity, precision, and F1-score. Among these four different models, ResNet 50 has proven to be the most effective choice with the highest accuracy (0.9572 for binary, 0.9474 for multi-class) and AUC (0.9958 for binary, 0.9836 for multi-class) in both multi-class and binary classification tasks. Although VGG 19 matched the accuracy of ResNet 50 in both tasks, ResNet 50 consistently outperformed it in terms of AUC, underscoring its superior effectiveness in identifying differentiated cells. Overall, our study demonstrated the capability to use a CNN approach to predict stem cell fate based on morphology changes, which will potentially provide insights for the application of cell-based therapy and advance our understanding of regenerative medicine.

Traditional crisis management approaches have often overlooked the role of community and nonprofit organizations. In this study, we investigated how nonprofits and communities contributed to problem-solving during crises through self-organization on social media. We applied social network analysis to 17,732 interactions using #TwitterFoodBank, a self-organized network emerged during the early stage of COVID-19 to address food insecurity. Our results highlighted the significant role of nonprofits in coordinating the community’s self-organized network. However, they also revealed a lack of coordination among these organizations in maintaining a viable network. These findings have valuable implications for nonprofits seeking to leverage the potential of online self-organized communities in crisis management.

The polarization of Λ and ¯Λ hyperons along the beam direction has been measured relative to the second and third harmonic event planes in isobar Ru+Ru and Zr+Zr collisions at √sNN=200 GeV. This is the first experimental evidence of the hyperon polarization by the triangular flow originating from the initial density fluctuations. The amplitudes of the sine modulation for the second and third harmonic results are comparable in magnitude, increase from central to peripheral collisions, and show a mild pT dependence. The azimuthal angle dependence of the polarization follows the vorticity pattern expected due to elliptic and triangular anisotropic flow, and qualitatively disagrees with most hydrodynamic model calculations based on thermal vorticity and shear induced contributions. The model results based on one of existing implementations of the shear contribution lead to a correct azimuthal angle dependence, but predict centrality and pT dependence that still disagree with experimental measurements. Thus, our results provide stringent constraints on the thermal vorticity and shear-induced contributions to hyperon polarization. Comparison to previous measurements at RHIC and the LHC for the second-order harmonic results shows little dependence on the collision system size and collision energy.

A young boy remembers fond childhood memories at his grandmother's house, where he learned the small things in life that truly mattered.

Disruptive technological changes, including carbon capture and storage, can have macroeconomic rebound effects that pose a threat to long term environmental sustainability when not accompanied by pollution taxes. The paper demonstrates that when the elasticity of intertemporal substitution is less than one, implementing a Pigouvian tax effectively stabilizes pollution emissions, regardless of technical and consumption elasticities of substitution. However, if the elasticity of intertemporal substitution exceeds one, flexibility in technical or consumption substitution could cause sustainable growth to falter. The policy implications concerning the role of subsidies for clean technology are discussed.

CuS and CuS-rGO nanocomposites were synthesized by the hydrothermal method. The synthesized CuS and rGO-CuS nanocomposite materials were physically characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM) and were evaluated electrochemically for supercapacitor applications. The specific capacitance of CuS was determined to be 207 F/g, 150 F/g, and 97 F/g at a current density of 0.5 A/g, 5 A/g, and 20 A/g, respectively. The rGO-CuS nanocomposite showed improved specific capacitance of 350 F/g, 251 F/g, and 149 F/g at a current density of 0.5 A/g, 5 A/g, and 20 A/g, respectively.

Globally, air pollution accounts for approximately one in eight deaths, and diarrheal diseases account for one in nine child deaths annually. Lead exposure contributes to concerns of heart disease, stroke, and developmental intellectual disability. Further, across the world, nearly 23 million people are displaced by extreme weather events each year, which have been exacerbated by climate change and contribute to physical and mental health implications for entire communities. These and many other environmentally related experiences and their subsequent health outcomes are not experienced equally by race, ethnicity, or income, with Black, Brown, and Indigenous communities and low-income communities repeatedly experiencing the heaviest of burdens. Due to a variety of historic and contemporary policy and planning decisions, these patterns of environmental injustice persist on local, national, and global scales. In response, environmental justice (EJ) is a social movement, as well as a belief that people of all backgrounds deserve access to clean air and water and a healthy community in which to thrive. This chapter heavily focuses on the USA and its environmental health inequities, policies, and historic EJ movement. However, in our globalized society, EJ is a global issue that must be addressed as such by the field of public health.

The Gona paleoanthropological field project in the Afar region of Ethiopia has long been associated with the earliest Oldowan stone tools. However, over the last 20 years, ongoing research at Gona has expanded its contributions considerably, producing fossils of Ardipithecus kadabba, Ardipithecus ramidus, and Homo erectus, as well as additional archaeological evidence of the earliest Oldowan and early Acheulean. Moreover, in the last few years, the Gona team has turned its attention to the younger deposits exposed in its study area and discovered a surprising number of Middle Stone Age (MSA) and Later Stone Age (LSA) archaeological sites, mostly from the Late Pleistocene to the early-middle Holocene. While any sedimentary sequence is incomplete by virtue of episodic depositional and erosional events over time, we are struck by the relative completeness of the archaeological sequence at Gona (Quade et al., 2008). The hominin fossils attract deserved attention, but the Gona project may ultimately be best known for preserving arguably the most extensive, detailed, and continuous Stone Age or Paleolithic archaeological sequence in the world, all contained within a small 25 km × 10 km area, approximately half of the total project area.

Human impacts on wild populations are numerous and extensive, degrading habitats and causing population declines across taxa. Though these impacts are often studied individually, wild populations typically face suites of stressors acting concomitantly, compromising the fitness of individuals and populations in ways poorly understood and not easily predicted by the effects of any single stressor. Developing understanding of the effects of multiple stressors and their potential interactions remains a critical challenge in environmental biology. Here, we focus on assessing the impacts of two prominent stressors affecting many organisms across the planet – elevated salinity (an increasingly common pollutant in freshwater habitats) and elevated temperature. We examined a suite of physiological traits and components of fitness across populations of wood frogs originating from ponds that differ in their proximity to roads and thus their legacy of exposure to road salt pollution. When experimentally exposed to road salt, wood frogs showed reduced survival, especially those from ponds adjacent to roads, and delayed time to metamorphosis. Family level effects mediated these outcomes, but high salinity generally eroded family level variance. When combined, exposure to both temperature and salt resulted in very low survival, and this effect was strongest in roadside populations. Taken together, these results suggest that temperature is an important stressor capable of exacerbating impacts from a prominent contaminant confronting many freshwater organisms in salinized habitats. More broadly, it appears likely that toxicity might often be underestimated in the absence of multi-stressor approaches.

Facultatively symbiotic corals provide important experimental models to explore the establishment, maintenance, and breakdown of the mutualism between corals and members of the algal family Symbiodiniaceae. The temperate coral Astrangia poculata is one such model as it is not only facultatively symbiotic, but also occurs across a broad temperature and latitudinal gradient. Here, we report the de novo chromosome-scale assembly and annotation of the A. poculata genome. Though widespread segmental/tandem duplications of genomic regions were detected, we did not find strong evidence of a whole genome duplication (WGD) event. Comparison of the gene arrangement between A. poculata and the tropical coral Acropora millepora revealed 56.38% of the orthologous genes were conserved in syntenic blocks despite ∼415 million years of divergence. Gene families related to sperm hyperactivation and innate immunity, including lectins, were found to contain more genes in A. millepora relative to A. poculata. Sperm hyperactivation in A. millepora is expected given the extreme requirements of gamete competition during mass spawning events in tropical corals, while lectins are important in the establishment of coral-algal symbiosis. By contrast, gene families involved in sleep promotion, feeding suppression, and circadian sleep/wake cycle processes were expanded in A. poculata. These expanded gene families may play a role in A. poculata’s ability to enter a dormancy-like state (“winter quiescence”) to survive freezing temperatures at the northern edges of the species’ range.

Purpose: As part of the development of a speech-in-noise screener, speech recognition was measured in quiet and in an interrupted noise for 59 children with typical development according to parent report. Method: There were 39 monolingual and 20 bilingual participants. A corpus of 107 words were presented by a male and female speaker and present in the following conditions: +3 dB signal-to- noise ratio (SNR), −3 dB SNR, and quiet. Results: The participants showed increasing difficulty with speech identification as the SNR decreased. Additionally, age-related differences in accuracy were observed at each noise level. Conclusion: Our findings provide preliminary support for the utility and efficacy of a speech-in-noise screener for use with children.

Innovation is needed to solve nursing workforce issues during times of crisis. A collaborative effort between a hospital system and several universities resulted in the Bridge to Professional Practice Program that was implemented during a period of high patient volume and nursing student downtime. The program provided support for staffing needs and clinical hours to promote readiness for practice for students. The program evaluation outcomes and recommendations for improvement are addressed.

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Recent advances suggest that shallow-mantle processes such as edge-driven conve...

Although clinical social work seeks to center the transformative potential of human relationships, practitioners are experiencing heightened systemic and organizational impingements from the dehumanizing pressures of neoliberalism. Neoliberalism and racism diminish the vitality and transformative potential of human relationships, disproportionately affecting Black, Indigenous and People of Color (BIPOC) communities. Practitioners are also experiencing increased stress and burnout related to increased caseloads and decreased professional autonomy and organizational practitioner support. Holistic, culturally responsive, and anti-oppressive processes seek to counter these oppressive forces but need further development to synthesize antioppressive structural understandings with embodied relational interactions. Practitioners can potentially contribute to efforts that apply critical theories and antioppressive understandings within their practice and workplace. Through an iterative flow of three sets of practices, the RE/UN/DIScover heuristic supports practitioners’ efforts to respond in those challenging everyday moments where oppressive forms of power are imposed and embedded within systemic processes. With themselves and other colleagues, practitioners engage in compassionate REcover practices; use curious, critical reflection to UNcover full understandings of power dynamics, impacts, and meanings; and draw on creative courage to DIScover and enact socially just and humanizing responses. This paper describes how practitioners can use the RE/UN/DIScover heuristic in two common challenging moments of clinical practice: systemic practice impingements and implementing a new training or practice model. The heuristic seeks to support practitioners’ efforts to preserve and expand socially just, relational spaces for themselves and those with whom they work within the context of systemic dehumanizing neoliberal forces.

The stratigraphic architecture of fjords is complicated due to the delicate interplay between ice dynamics, sediment supply, relative sea-level fluctuations and slope failures. Glaciogenic sediment is prone to failure and to be carried downslope to the fjord floor through the entire spectrum of mass movements and subaqueous density flows, as the unstable paraglacial submarine landscape moves towards stability. Palaeofjords formed by Gondwanan glaciers during the late Palaeozoic Ice Age contain a compelling record of gravitational resedimentation in fjord depositional systems. This paper showcases the geomorphology and depositional history of a glacial cycle in the Orutanda fjord in north-western Namibia as an example of an overdeepened fjord basin fill dominated by products of subaqueous gravitational processes. During glaciation, the Orutanda glacier carved a 20 km long by 3.7 km wide glacial trough that embodies an overdeepened basin. Ice thickness during terminal glacial occupation of the fjord is estimated to had been up to 200 m based on the fjord geomorphology. The progressive retreat of the tidewater glacier, concomitant with marine flooding, increased accommodation space in the overdeepened basin during deglaciation. During this stage, proglacial sedimentation through iceberg rafting and settling of turbid plumes was outpaced by intense paraglacial downslope resedimentation of glacially-transported debris. Successive failures from the fjord walls and downslope resedimentation resulted in coalescing debrite–turbidite lobes on the fjord floor. Slide deposits, composed entirely of deformed debrites and turbidites, indicate that these resedimented facies were prone to renewed mass wasting. As the Orutanda glacier melted, the fjord experienced the axial progradation of a fjord-head delta registered only by turbidites and slide deposits derived from its collapse. The Orutanda fjord sheds light on the relevance of paraglacial mass wasting in overprinting glaciogenic deposits. This insight is key to understanding the role of glaciers versus non-glacial processes in producing the glacial deep-time record.

The academic accounting labor market is experiencing rapid changes on several fronts. We examine accounting program leaders’ perceptions of Assistant Professor hiring criteria, including how the criteria have changed in recent years. We ask program leaders to provide qualitative perspectives, rate the importance of 56 possible hiring criteria, and, in a supplemental analysis, rank and rate four hypothetical Assistant Professor candidates. Based on responses from 54 accounting program leaders, the results reveal a complex setting with research and teaching considerations, a focus on collegiality and professional experience, and an increasing focus on diversity. Specifically, Research Intensive schools (Carnegie R1 and/or doctorate in business) are more top-tier research and top-tier doctoral program focused, while Non-Research Intensive schools (all others) are more focused on general skills, other refereed journals, practitioner research, teaching experience, service, CPA licensure, and other professional certifications.

The Late Paleozoic Ice Age (LPIA) was the last major icehouse climate (low CO2,...

Course-based research pedagogy involves positioning students as contributors to authentic research projects as part of an engaging educational experience that promotes their learning and persistence in science. To develop a model for assessing and grading students engaged in this type of learning experience, the assessment aims and practices of a community of experienced course-based research instructors were collected and analyzed. This approach defines four aims of course-based research assessment—(1) Assessing Laboratory Work and Scientific Thinking; (2) Evaluating Mastery of Concepts, Quantitative Thinking and Skills; (3) Appraising Forms of Scientific Communication; and (4) Metacognition of Learning—along with a set of practices for each aim. These aims and practices of assessment were then integrated with previously developed models of course-based research instruction to reveal an assessment program in which instructors provide extensive feedback to support productive student engagement in research while grading those aspects of research that are necessary for the student to succeed. Assessment conducted in this way delicately balances the need to facilitate students’ ongoing research with the requirement of a final grade without undercutting the important aims of a CRE education.